Cytokeratin 19 抗体
Quick Overview for Cytokeratin 19 抗体 (ABIN3025844)
抗原
See all Cytokeratin 19 (KRT19) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human KRT19 protein was used as the immunogen for the CK19 antibody.
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亚型
- IgG1 kappa
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anti-Keratin 19 (KRT19) antibody
KRT19 适用: 人 WB, ELISA, IHC (p), ICC, IP, ICFC 宿主: 小鼠 Monoclonal A53-B-A2 unconjugated
anti-Keratin 19 (KRT19) (AA 245-391) antibodyKRT19 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 19 (KRT19) (AA 245-391) antibodyKRT19 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D1 unconjugated
anti-Keratin 19 (KRT19) (AA 1-403) antibodyKRT19 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 19 (KRT19) (AA 127-322) antibodyKRT19 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 适用: 人, 小鼠, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal KRT19-800 unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 适用: 人 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal KRT19-799 unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 适用: 人 WB, IHC (p), ICC, IP, ICFC 宿主: 小鼠 Monoclonal BA-17 unconjugated
anti-Keratin 19 (KRT19) (AA 241-400) antibodyKRT19 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 19 (KRT19) (AA 274-400) antibodyKRT19 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 8H1A8 unconjugated
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应用备注
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Optimal dilution of the CK19 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CK19 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
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别名
- CK19
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背景
- Recognizes a protein of 40 kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
抗原
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