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Liver Arginase 抗体 (AA 1-150)

This anti-Liver Arginase antibody is a 小鼠 单克隆 antibody detecting Liver Arginase in WB, FACS, IHC (p) 和 IF. Suitable for 人.
产品编号 ABIN3025822
发货至: 中国

Quick Overview for Liver Arginase 抗体 (AA 1-150) (ABIN3025822)

抗原

See all Liver Arginase (ARG1) 抗体
Liver Arginase (ARG1) (Arginase, Liver (ARG1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Liver Arginase antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

克隆位点

ARG1-1125
  • 抗原表位

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    AA 1-150

    纯化方法

    Protein G affinity chromatography

    免疫原

    A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody.

    亚型

    IgG3 kappa
  • 应用备注

    Optimal dilution of the Arginase 1 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    0.2 mg/mL

    缓冲液

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Arginase 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    Liver Arginase (ARG1) (Arginase, Liver (ARG1))

    别名

    Arginase 1

    背景

    Recognizes a protein of 35-38  kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

    途径

    Cellular Response to Molecule of Bacterial Origin
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