Nuclear Antigen 抗体
Quick Overview for Nuclear Antigen 抗体 (ABIN3025764)
抗原
See all Nuclear Antigen (IPO-38) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- PEG precipitation
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免疫原
- Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO-38 antibody.
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亚型
- IgM kappa
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anti-Nuclear Antigen (IPO-38) antibody
IPO-38 适用: 人, 小鼠, 大鼠 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal SPM260 unconjugated
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应用备注
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Optimal dilution of the IPO-38 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the IPO-38 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Nuclear Antigen (IPO-38)
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别名
- IPO-38
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背景
- Recognizes a protein of 14-16 kDa, which is a novel nuclear antigen of proliferating cells. IPO38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO38 may be a useful marker of cell proliferation during monitoring of tumor progression.
抗原
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