MYOD1 抗体 (AA 180-189)
Quick Overview for MYOD1 抗体 (AA 180-189) (ABIN3025672)
抗原
See all MYOD1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 180-189
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant mouse protein was used as the immunogen for this anti-MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189.
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亚型
- IgG1 kappa
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anti-Myogenic Differentiation 1 (MYOD1) (AA 150-250) antibody
MYOD1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 51-150) antibodyMYOD1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) antibodyMYOD1 适用: 人 IHC, StM 宿主: 兔 Monoclonal MYOD1-2075R unconjugated Recombinant Antibody
anti-Myogenic Differentiation 1 (MYOD1) antibodyMYOD1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rMYD712 unconjugated Recombinant Antibody
anti-Myogenic Differentiation 1 (MYOD1) (AA 1-320) antibodyMYOD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) antibodyMYOD1 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 211-320) antibodyMYOD1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 1-320) antibodyMYOD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 211-320) antibodyMYOD1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 211-240) antibodyMYOD1 适用: 人 WB, IF, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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The optimal dilution of the anti-MyoD1 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-MyoD1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- MYOD1 (Myogenic Differentiation 1 (MYOD1))
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别名
- MyoD1
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背景
- Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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