CA9 抗体
Quick Overview for CA9 抗体 (ABIN3025645)
抗原
See all CA9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX antibody.
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亚型
- IgG2b kappa
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anti-Carbonic Anhydrase IX (CA9) (AA 37-186) antibody
CA9 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7C4A5 unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 59-414) antibodyCA9 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 37-186) antibodyCA9 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 10F7A8 unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 52-151) antibodyCA9 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 113-143) antibodyCA9 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 251-350) antibodyCA9 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 9B5 unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人 WB, IP 宿主: 小鼠 Monoclonal 12F10 unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人, 马 WB, IHC, StM 宿主: 小鼠 Monoclonal CA9-781 unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 113-143) antibodyCA9 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB18383 unconjugated
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应用备注
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The optimal dilution of the Carbonic Anhydrase IX antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Carbonic Anhydrase IX antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CA9 (Carbonic Anhydrase IX (CA9))
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别名
- Carbonic Anhydrase IX
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背景
- Recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/CA9/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This mAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
抗原
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