WT1 抗体
Quick Overview for WT1 抗体 (ABIN3025552)
抗原
See all WT1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- A recombinant human protein was used as the immunogen for the WT1 antibody.
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亚型
- IgG1 kappa
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anti-Wilms Tumor 1 (WT1) (AA 1-302) antibody
WT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) antibodyWT1 适用: 人 IHC, WB 宿主: 兔 Monoclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 适用: 人, 猴 IHC, FACS, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 7F9E8 unconjugated
anti-Wilms Tumor 1 (WT1) antibodyWT1 适用: 人, 大鼠, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal rWT1-857 unconjugated Recombinant Antibody
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 适用: 人, 猴 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 7F9G7H2 unconjugated
anti-Wilms Tumor 1 (WT1) (C-Term) antibodyWT1 适用: 人, 大鼠, 小鼠, 兔, 犬, 猴, Bat, 小鸡, 非洲爪蟾 IHC, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 适用: 人, 大鼠, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal 6F-H2 unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-302) antibodyWT1 适用: 人 IF, WB 宿主: 兔 Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 314-479) antibodyWT1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 5G11A5 unconjugated
anti-Wilms Tumor 1 (WT1) (AA 346-375) antibodyWT1 适用: 人, 小鼠 IF, FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB18841 unconjugated
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应用备注
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Optimal dilution of the WT1 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted format: incubate for 30 min at RT (1) -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the WT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- WT1 (Wilms Tumor 1 (WT1))
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别名
- WT1
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背景
- Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
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途径
- Tube Formation
抗原
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