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- 抗原 See all WT1 抗体
- WT1 (Wilms Tumor 1 (WT1))
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抗原表位
- AA 1-181
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This WT1 antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- A recombinant protein corresponding to residues 1-181 from the human protein was used as the immunogen for the Wilms Tumor 1 antibody.
- 克隆位点
- 6F-H2
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product WT1 Primary Antibody
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anti-Wilms Tumor 1 (WT1) (AA 1-302) antibody
WT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) antibodyWT1 适用: 人 IHC, WB 宿主: 兔 Monoclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 适用: 人 FACS, IHC, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 7F9E8 unconjugated
anti-Wilms Tumor 1 (WT1) antibodyWT1 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal rWT1-857 unconjugated Recombinant Antibody
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 7F9G7H2 unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal 6F-H2 unconjugated
anti-Wilms Tumor 1 (WT1) (C-Term) antibodyWT1 适用: 人, 小鼠, 大鼠, 犬, 兔, Bat, 小鸡, 猴, 非洲爪蟾 IHC, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-302) antibodyWT1 适用: 人 IF, WB 宿主: 兔 Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 314-479) antibodyWT1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 5G11A5 unconjugated
anti-Wilms Tumor 1 (WT1) (AA 346-375) antibodyWT1 适用: 人, 小鼠 IF, FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB18841 unconjugated
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- 应用备注
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Optimal dilution of the Wilms Tumor 1 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Wilms Tumor 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- WT1 (Wilms Tumor 1 (WT1))
- 别名
- Wilms Tumor 1 (WT1 产品)
- 背景
- Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
- 途径
- Tube Formation
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