S100A9 抗体
Quick Overview for S100A9 抗体 (ABIN3025524)
抗原
See all S100A9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- PEG precipitation
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免疫原
- Recombinant human protein was used as the immunogen for the S100A9 antibody.
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亚型
- IgM kappa
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anti-S100 Calcium Binding Protein A9 (S100A9) (Internal Region) antibody
Verified S100A9 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) antibodyS100A9 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) antibodyS100A9 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) antibodyS100A9 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 51-114) antibodyS100A9 适用: 人 ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) antibodyS100A9 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1C22 unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) antibodyS100A9 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C10 unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) antibodyS100A9 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal S100A9-1075 unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 2-113) antibodyS100A9 适用: 大鼠, 小鼠 WB, IHC, ELISA, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 2-114) antibodyS100A9 适用: 人 WB, IHC, ELISA, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the S100A9 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Formalin-fixed): 0.5-1 μg/mL for 30 min at RT (1),Not suitable for frozen tissues
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the S100A9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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别名
- S100A9
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背景
- Recognizes a 14 kDa protein, identified as S100A9 (also known as Calgranulin B or MRP14), expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This mAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
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途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
抗原
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