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S100A8/A9 Complex (Calprotectin) 抗体

This 小鼠 单克隆 antibody specifically detects S100A8/A9 Complex (Calprotectin) in FACS, IF 和 IHC (p). It exhibits reactivity toward 人.
产品编号 ABIN3025523
发货至: 中国
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Quick Overview for S100A8/A9 Complex (Calprotectin) 抗体 (ABIN3025523)

抗原

See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) 抗体
S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This S100A8/A9 Complex (Calprotectin) antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

CAGB-426
  • 纯化方法

    PEG precipitation

    免疫原

    Recombinant human protein was used as the immunogen for the Calgranulin A + B antibody.

    亚型

    IgM kappa
  • 应用备注

    Optimal dilution of the Calgranulin A + B antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Formalin-fixed): 0.5-1 μg/mL for 30 min at RT (1),Not suitable for frozen tissues

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Calgranulin A + B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

    别名

    Calgranulin A + B

    背景

    Recognizes a 12-14  kDa doublet of Calgranulin A/B (also known as S100A8/A9 or MRP-8/14), expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

    途径

    S100 Proteins
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