PAX6 抗体 (AA 1-300)
Quick Overview for PAX6 抗体 (AA 1-300) (ABIN3025374)
抗原
See all PAX6 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-300
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纯化方法
- Protein G affinity chromatography
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免疫原
- A recombinant fragment (N-terminus, aa 1-300) was used as the immunogen for the PAX6 antibody.
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亚型
- IgG1 kappa
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anti-Paired Box 6 (PAX6) (AA 1-122) antibody
PAX6 适用: 人 ELISA, FACS, IHC 宿主: 小鼠 Monoclonal 1F6G2 unconjugated
anti-Paired Box 6 (PAX6) (AA 51-150) antibodyPAX6 适用: 人, 小鼠, 大鼠 ELISA, FACS, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Paired Box 6 (PAX6) (AA 183-210) antibodyPAX6 适用: 人 WB, IF, ELISA, FACS, IHC 宿主: 兔 Polyclonal unconjugated
anti-Paired Box 6 (PAX6) (AA 352-380) antibodyPAX6 适用: 人 WB, IF, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Paired Box 6 (PAX6) (AA 1-422) antibodyPAX6 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Paired Box 6 (PAX6) (AA 195-233), (Middle Region) antibodyPAX6 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Paired Box 6 (PAX6) (AA 352-380) antibodyPAX6 适用: 人, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB15517 unconjugated
anti-Paired Box 6 (PAX6) (AA 1-300) antibodyPAX6 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Paired Box 6 (PAX6) (AA 1-422) antibodyPAX6 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Paired Box 6 (PAX6) antibodyPAX6 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-706M unconjugated
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应用备注
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Optimal dilution of the PAX6 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the PAX6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- PAX6 (Paired Box 6 (PAX6))
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别名
- PAX6
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背景
- Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.
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途径
- Peptide Hormone Metabolism, Carbohydrate Homeostasis
抗原
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