CD43 抗体
Quick Overview for CD43 抗体 (ABIN3025352)
抗原
See all CD43 (SPN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- PEG precipitation
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免疫原
- Immature pluripotent human leukemia K562 cells were used as the immunogen for the CD43 antibody.
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亚型
- IgM kappa
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应用备注
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Optimal dilution of the CD43 antibody should be determined by the researcher.
1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1 mg/mL PBS, 15 min at room temp
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CD43 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD43 (SPN) (Sialophorin (SPN))
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别名
- CD43
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背景
- One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt]
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途径
- Regulation of Leukocyte Mediated Immunity
抗原
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