Myogenin 抗体 (full length)
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北京 101111
Quick Overview for Myogenin 抗体 (full length) (ABIN3025312)
抗原
See all Myogenin (MYOG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 30-224, full length
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原理
- Myogenin Antibody Cocktail
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纯化方法
- Protein G affinity chromatography
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免疫原
- Full length human recombinant protein (MGN185) and a partial rat recombinant protein (amino acid 30-224) (F5D) were used as the immunogens for this Myogenin antibody cocktail.
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亚型
- IgG1, kappa
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应用备注
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The optimal dilution of the Myogenin antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Myogenin antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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别名
- Myogenin
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背景
- Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
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UniProt
- P15173
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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