Myogenin 抗体 (full length)
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- 抗原 See all Myogenin (MYOG) 抗体
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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抗原表位
- AA 30-224, full length
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适用
- 人, 小鼠, 大鼠, Pig, Cat
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Myogenin antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Full length human recombinant protein (MGN185) and a partial rat recombinant protein (amino acid 30-224) (F5D) were used as the immunogens for this Myogenin antibody cocktail.
- 克隆位点
- MGN185-F5D
- 亚型
- IgG1
- Top Product
- Discover our top product MYOG Primary Antibody
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anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 4-219) antibody
MYOG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2B7 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) antibodyMYOG 适用: 人, 小鼠, 大鼠, Pig, Cow, 小鸡 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E7 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E19 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-30) antibodyMYOG 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (Internal Region) antibodyMYOG 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (Internal Region) antibodyMYOG 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3E3 unconjugated
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- 应用备注
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The optimal dilution of the Myogenin antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Myogenin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
- 别名
- Myogenin (MYOG 产品)
- 背景
- Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
- 途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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