MVP 抗体
Quick Overview for MVP 抗体 (ABIN3025305)
抗原
See all MVP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the Major Vault Protein antibody.
-
亚型
- IgG1 kappa
-
-
-
-
应用备注
-
Optimal dilution of the Major Vault Protein antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
-
储存液
- Azide free
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store the Major Vault Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
-
- MVP (Major Vault Protein (MVP))
-
别名
- Major Vault Protein
-
背景
- MVP is required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
抗原
-