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MVP 抗体

This anti-MVP antibody is a 小鼠 单克隆 antibody detecting MVP in WB, FACS, IF 和 IHC (p). Suitable for 人.
产品编号 ABIN3025305
发货至: 中国

Quick Overview for MVP 抗体 (ABIN3025305)

抗原

See all MVP 抗体
MVP (Major Vault Protein (MVP))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This MVP antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

1032
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the Major Vault Protein antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Optimal dilution of the Major Vault Protein antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Major Vault Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    MVP (Major Vault Protein (MVP))

    别名

    Major Vault Protein

    背景

    MVP is required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
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