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MUC6 抗体

This anti-MUC6 antibody is a 小鼠 单克隆 antibody detecting MUC6 in IF, WB, FACS 和 IHC (p). Suitable for 人.
产品编号 ABIN3025254
发货至: 中国

Quick Overview for MUC6 抗体 (ABIN3025254)

抗原

See all MUC6 抗体
MUC6 (Mucin 6, Oligomeric Mucus/gel-Forming (MUC6))

适用

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宿主

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小鼠

克隆类型

  • 45
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单克隆

标记

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This MUC6 antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

MUC6-916
  • 无交叉反应

    大鼠

    纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human protein was used as the immunogen for the Mucin-6 antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Optimal dilution of the Mucin-6 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    0.2 mg/mL

    缓冲液

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Mucin-6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    MUC6 (Mucin 6, Oligomeric Mucus/gel-Forming (MUC6))

    别名

    Mucin-6

    背景

    MUC6 or gastric mucin is a secreted glycoprotein that plays an essential role in epithelial cyto-protection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileum and right colon. In gastric cancer, Mucin 6 has an altered expression. In normal stomach, Mucin 6 is associated with Lewis type 2, Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deeper mucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation.
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