MITF 抗体 (N-Term)
Quick Overview for MITF 抗体 (N-Term) (ABIN3025233)
抗原
See all MITF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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无交叉反应
- 小鼠, 大鼠
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纯化方法
- Protein G affinity chromatography
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免疫原
- An N-terminus fragment of human Mi protein was used as the immunogen for the MITF antibody.
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亚型
- IgG1 kappa
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anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 351-450) antibody
MITF 适用: 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (pSer180), (pSer73) antibodyMITF 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-114) antibodyMITF 适用: 人 WB, FACS, ELISA 宿主: 小鼠 Monoclonal 8F1G5 unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-28), (N-Term) antibodyMITF 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB18810 unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibodyMITF 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人, 犬 IHC, IF, FACS, StM 宿主: 兔 Monoclonal MITF-2987R unconjugated Recombinant Antibody
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-280) antibodyMITF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibodyMITF 适用: 人, 小鼠 WB, IHC, ELISA, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal D5 unconjugated
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应用备注
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Optimal dilution of the MITF antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the MITF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- MITF (Microphthalmia-Associated Transcription Factor (MITF))
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别名
- MITF
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背景
- MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
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途径
- Chromatin Binding
抗原
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