ACP5 抗体
Quick Overview for ACP5 抗体 (ABIN3025074)
抗原
See all ACP5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- It recognizes a protein of 35 kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This mAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher s Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant full-length human protein was used as the immunogen for the TRAcP antibody.
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亚型
- IgG2b kappa
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anti-Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 221-325) antibody
ACP5 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5C5E7 unconjugated
anti-Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 221-325) antibodyACP5 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7E6A11 unconjugated
anti-Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 76-325) antibodyACP5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 221-325) antibodyACP5 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 29-296) antibodyACP5 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 1-325) antibodyACP5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 72-158) antibodyACP5 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2D9 unconjugated
anti-Acid Phosphatase 5, Tartrate Resistant (ACP5) antibodyACP5 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal rACP5-1070 unconjugated Recombinant Antibody
anti-Acid Phosphatase 5, Tartrate Resistant (ACP5) (N-Term) antibodyACP5 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 74-321) antibody (APC)ACP5 适用: 兔 WB, IHC, IP, ICC 宿主: 豚鼠 Polyclonal APC
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应用备注
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Optimal dilution of the TRAcP antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the TRAcP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))
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别名
- TRAcP
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背景
- It recognizes a protein of 35 kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This mAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher s Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.
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途径
- Transition Metal Ion Homeostasis
抗原
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