AMPD3 抗体
Quick Overview for AMPD3 抗体 (ABIN3025068)
抗原
See all AMPD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- It recognizes a protein of ~90 kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This mAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This mAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant full-length human protein was used as the immunogen for the AMPD3 antibody.
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亚型
- IgG2b kappa
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anti-Adenosine Monophosphate Deaminase 3 (AMPD3) antibody
AMPD3 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal AMPD3-901 unconjugated
anti-Adenosine Monophosphate Deaminase 3 (AMPD3) (AA 1-260) antibodyAMPD3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Monophosphate Deaminase 3 (AMPD3) (AA 325-356) antibodyAMPD3 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB17671 unconjugated
anti-Adenosine Monophosphate Deaminase 3 (AMPD3) antibodyAMPD3 适用: 人 IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the AMPD3 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 1-2 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunocytochemistry: 1-2 μg/mL for 30 min at RT,Immunohistology (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the AMPD3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- AMPD3 (Adenosine Monophosphate Deaminase 3 (AMPD3))
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别名
- AMPD3
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背景
- It recognizes a protein of ~90 kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This mAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This mAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.
抗原
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