The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GM-CSF antibody to be titered up or down for optimal performance.\. Functional Activity (Neutralization): neutralizes GM-CSF bioactivity,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1 μg/mL,IP: 0.5-1 μg/500 μg protein lysate,IHC (Frozen): 0.5-1 μg/mL for 30 minutes at RT
限制
仅限研究用
浓度
1 mg/mL
缓冲液
1 mg/mL in 1X PBS, BSA free, sodium azide free
储存液
Azide free
储存条件
4 °C,-20 °C
储存方法
Store the GM-CSF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
GM-CSF (Granulocyte/macrophage - Colony-stimulating factor) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.