Glypican 3 抗体
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- 抗原 See all Glypican 3 (GPC3) 抗体
- Glypican 3 (GPC3)
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适用
- 人, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Glypican 3 antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant full-length human GPC3 protein was used as the immunogen for the Glypican-3 antibody.
- 克隆位点
- GPC3-863
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product GPC3 Primary Antibody
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anti-Glypican 3 (GPC3) antibody
GPC3 适用: 人, 小鼠 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 9C2 unconjugated
anti-Glypican 3 (GPC3) (AA 344-559) antibodyGPC3 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Glypican 3 (GPC3) antibodyGPC3 适用: 人, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal GPC3-863 unconjugated
anti-Glypican 3 (GPC3) (AA 55-200) antibodyGPC3 适用: 人 IF, FACS, WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 9C2 unconjugated
anti-Glypican 3 (GPC3) (AA 55-200) antibodyGPC3 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2A2C3 unconjugated
anti-Glypican 3 (GPC3) (AA 359-554) antibodyGPC3 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2C7E9 unconjugated
anti-Glypican 3 (GPC3) antibodyGPC3 适用: 人, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal rGPC3-863 unconjugated Recombinant Antibody
anti-Glypican 3 (GPC3) (AA 511-580) antibodyGPC3 适用: 人 IF, FACS, WB, IHC, StM 宿主: 小鼠 Monoclonal 1G12 unconjugated
anti-Glypican 3 (GPC3) (AA 359-554) antibodyGPC3 适用: 人, 大鼠 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 4D5B1 unconjugated
anti-Glypican 3 (GPC3) antibodyGPC3 适用: 人, 小鼠 IF, WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 9C2 unconjugated
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- 应用备注
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Optimal dilution of the Glypican-3 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Glypican-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Glypican 3 (GPC3)
- 别名
- Glypican-3 (GPC3 产品)
- 别名
- GPC3 antibody, sgb antibody, dgsx antibody, sdys antibody, sgbs antibody, oci-5 antibody, sgbs1 antibody, DGSX antibody, GTR2-2 antibody, MXR7 antibody, OCI-5 antibody, SDYS antibody, SGB antibody, SGBS antibody, SGBS1 antibody, Glypican-3 antibody, glypican 3 antibody, gpc3 antibody, GPC3 antibody, Gpc3 antibody
- 背景
- Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100 % in follicular carcinoma and 70 % in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
- 途径
- Glycosaminoglycan Metabolic Process
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