Fascin 抗体
Quick Overview for Fascin 抗体 (ABIN3024845)
抗原
See all Fascin (FSCN1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Full length recombinant human protein was used as the immunogen for the Fascin antibody.
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亚型
- IgG2b kappa
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anti-Fascin (FSCN1) antibody
FSCN1 适用: 人 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal FSCN1-417 unconjugated
anti-Fascin (FSCN1) antibodyFSCN1 适用: 人, 大鼠 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal FSCN1-418 unconjugated
anti-Fascin (FSCN1) (AA 11-110) antibodyFSCN1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fascin (FSCN1) antibodyFSCN1 适用: 人 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal FSCN1-416 unconjugated
anti-Fascin (FSCN1) (AA 394-493) antibodyFSCN1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Fascin (FSCN1) (pSer39) antibodyFSCN1 适用: 人, 小鼠 WB, FACS, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fascin (FSCN1) (AA 322-350) antibodyFSCN1 适用: 人 WB, IHC, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fascin (FSCN1) (AA 2-187) antibodyFSCN1 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fascin (FSCN1) (AA 1-493) antibodyFSCN1 适用: 人 WB, FACS, IF, ELISA, ICC 宿主: 小鼠 Monoclonal AT13D2 unconjugated
anti-Fascin (FSCN1) (AA 93-142) antibodyFSCN1 适用: 人, 小鼠, 大鼠, 豚鼠, 犬, 马, 斑马鱼, 猴, Pig, Hamster WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the Fascin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistology (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Fascin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Fascin (FSCN1)
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别名
- Fascin
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背景
- Fascin/FSCN1 binds beta-catenin and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of FSCN1 in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. It localizes to actin-rich protrusions at the cell surface called filopodia. FSCN1 localizes to invadopodia, membrane protrusions formed at the adherent cell surface that facilitate extracellular matrix (ECM) invasion, this provide a potential molecular mechanism for how fascin increases the invasiveness of cancer cells since fascin expression is upregulated in a spectrum of cancers. [Wiki]
抗原
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