ANO1 抗体 (AA 904-986)
Quick Overview for ANO1 抗体 (AA 904-986) (ABIN3024818)
抗原
See all ANO1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 904-986
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids 904-986 from the human protein was used as the immunogen for this DOG-1 antibody.
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亚型
- IgG1 kappa
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anti-Anoctamin 1, Calcium Activated Chloride Channel (ANO1) (AA 151-250) antibody
ANO1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Anoctamin 1, Calcium Activated Chloride Channel (ANO1) (AA 20-239) antibodyANO1 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Anoctamin 1, Calcium Activated Chloride Channel (ANO1) (AA 1-151) antibodyANO1 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Anoctamin 1, Calcium Activated Chloride Channel (ANO1) (AA 2-101) antibodyANO1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal DG1-1484 unconjugated
anti-Anoctamin 1, Calcium Activated Chloride Channel (ANO1) (AA 2-101) antibodyANO1 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-201M unconjugated
anti-Anoctamin 1, Calcium Activated Chloride Channel (ANO1) (Middle Region) antibodyANO1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Anoctamin 1, Calcium Activated Chloride Channel (ANO1) (AA 2-101) antibodyANO1 适用: 人 IHC, StM 宿主: 兔 Monoclonal DG1-2564R unconjugated Recombinant Antibody
anti-Anoctamin 1, Calcium Activated Chloride Channel (ANO1) antibodyANO1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rDG1-447 unconjugated Recombinant Antibody
anti-Anoctamin 1, Calcium Activated Chloride Channel (ANO1) (Internal Region) antibodyANO1 适用: 人, 小鼠, 大鼠 IHC, IF, WB, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Anoctamin 1, Calcium Activated Chloride Channel (ANO1) (AA 628-731) antibodyANO1 适用: 人 IF, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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The optimal dilution of the DOG-1 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the DOG-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))
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别名
- DOG-1
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背景
- Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4 % vs. 94.7 %.
抗原
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