EpCAM 抗体 (AA 27-59)
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Quick Overview for EpCAM 抗体 (AA 27-59) (ABIN3024734)
抗原
See all EpCAM (EPCAM) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 27-59
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原理
- EpCAM Antibody (azide and preservative free)
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无交叉反应
- 大鼠
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纯化方法
- Protein G affinity chromatography
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免疫原
- Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for this EpCAM antibody. The epitope has been localized to amino acids 27-59.
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亚型
- IgG1, kappa
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应用备注
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The optimal dilution of the EpCAM antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- EpCAM
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背景
- EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
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UniProt
- P16422
抗原
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