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EpCAM 抗体 (Cytoplasmic Domain)

EPCAM 适用: 人, 小鼠, 大鼠 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal EGP40-1110 unconjugated
产品编号 ABIN3024723
发货至: 中国
  • 抗原 See all EpCAM (EPCAM) 抗体
    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
    抗原表位
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    Cytoplasmic Domain
    适用
    • 276
    • 74
    • 34
    • 2
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    人, 小鼠, 大鼠
    宿主
    • 144
    • 142
    • 10
    • 1
    小鼠
    克隆类型
    • 179
    • 114
    • 2
    单克隆
    标记
    • 149
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    This EpCAM antibody is un-conjugated
    应用范围
    • 214
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    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    A recombinant human protein fragment from the cytoplasmic domain was used as the immunogen for the EpCAM antibody.
    克隆位点
    EGP40-1110
    亚型
    IgG2b kappa
    Top Product
    Discover our top product EPCAM Primary Antibody
  • 应用备注
    Optimal dilution of the EpCAM antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    0.2 mg/mL
    缓冲液
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
    别名
    EpCAM (EPCAM 产品)
    别名
    DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody
    背景
    EGP40 is a 40-43  kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
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