EpCAM 抗体
Quick Overview for EpCAM 抗体 (ABIN3024720)
抗原
See all EpCAM (EPCAM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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无交叉反应
- 人
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纯度
- Unpurified high titer supernatant
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免疫原
- Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.
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亚型
- IgG1 kappa
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应用备注
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Optimal dilution of the rat EpCAM antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The epitopes of mAbs Epcam/1158 and < a href=../tds/rat-epcam-antibody-epcam-1159-v3118s>Epcam/1159 monoclonal antibodies are different.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 5-10 μL/million cells in 0.1ml,Immunofluorescence: 1:50-1:100,Western blot: 1:100-1:200,Immunohistochemistry (FFPE): 1:50-1:100 30 min at RT (1),Prediluted format: incubate for 30 min at RT (3) -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Bioreactor concentrate with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- Store the rat EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- EpCAM
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背景
- EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
抗原
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