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EpCAM 抗体

The 小鼠 单克隆 anti-EpCAM antibody is suitable to detect EpCAM in samples from 小鼠 和 大鼠. It has been validated for WB, FACS, IF 和 IHC (p).
产品编号 ABIN3024720
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for EpCAM 抗体 (ABIN3024720)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

适用

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小鼠

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单克隆

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This EpCAM antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

Epcam-1158
  • 无交叉反应

    纯度

    Unpurified high titer supernatant

    免疫原

    Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Optimal dilution of the rat EpCAM antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The epitopes of mAbs Epcam/1158 and < a href=../tds/rat-epcam-antibody-epcam-1159-v3118s>Epcam/1159 monoclonal antibodies are different.
    3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 5-10 μL/million cells in 0.1ml,Immunofluorescence: 1:50-1:100,Western blot: 1:100-1:200,Immunohistochemistry (FFPE): 1:50-1:100 30 min at RT (1),Prediluted format: incubate for 30 min at RT (3)

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Bioreactor concentrate with 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C,4 °C

    储存方法

    Store the rat EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    EpCAM

    背景

    EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
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