CERK 抗体 (Internal Region)
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Quick Overview for CERK 抗体 (Internal Region) (ABIN302472)
抗原
See all CERK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- This antibody detects Ceramide Kinase (CERK) at internal domain.
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纯化方法
- Immunoaffinity Chromatography
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免疫原
- CERK antibody was raised against synthetic peptide from internal domain of human CERK.
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anti-Ceramide Kinase (CERK) (AA 212-478) antibody
CERK 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ceramide Kinase (CERK) (AA 487-516), (C-Term) antibodyCERK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB5271 unconjugated
anti-Ceramide Kinase (CERK) antibodyCERK 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ceramide Kinase (CERK) (AA 1-201) antibodyCERK 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ceramide Kinase (CERK) (AA 1-201) antibodyCERK 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ceramide Kinase (CERK) (AA 487-516) antibodyCERK 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ceramide Kinase (CERK) (C-Term) antibodyCERK 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry on paraffin sections (20 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS containing 0.09 % sodium azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.
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- CERK (Ceramide Kinase (CERK))
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别名
- Ceramide Kinase
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背景
- CERK, which phosphorylates Cer to produce ceramide 1-phosphate (C1P), was initially described as a Ca2+-stimulated lipid kinase that copurified with brain synaptic vesicles. CERK activity has been reported in HL60 cells, mast cells, and neutrophils. CERK is involved in phagolysosome formation in polymorphonuclear leukocytes and also in liposome fusion. C1P has been reported to have mitogenic effects. More recently, C1P was found to induce arachidonic acid release and prostanoid synthesis.Synonyms: Acylsphingosine kinase, KIAA1646, LK4, Lipid kinase 4, hCERK
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基因ID
- 64781
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NCBI登录号
- NP_073603
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UniProt
- Q8TCT0
抗原
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