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FBXO5 抗体 (N-Term)

FBXO5 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal FBXP5 unconjugated
产品编号 ABIN3024714
发货至: 中国
  • 抗原 See all FBXO5 抗体
    FBXO5 (F-Box Protein 5 (FBXO5))
    抗原表位
    • 12
    • 7
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    • 1
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    N-Term
    适用
    • 44
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    • 2
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    宿主
    • 27
    • 17
    小鼠
    克隆类型
    • 24
    • 20
    单克隆
    标记
    • 20
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    This FBXO5 antibody is un-conjugated
    应用范围
    • 35
    • 17
    • 12
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    • 5
    • 1
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.
    克隆位点
    FBXP5
    亚型
    IgG2a kappa
    Top Product
    Discover our top product FBXO5 Primary Antibody
  • 应用备注
    Optimal dilution of the EMI-1 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the EMI-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    FBXO5 (F-Box Protein 5 (FBXO5))
    别名
    EMI-1 (FBXO5 产品)
    别名
    EMI1 antibody, FBX5 antibody, Fbxo31 antibody, 2510044I10Rik antibody, C85305 antibody, Emi1 antibody, emi1 antibody, fc65h02 antibody, wu:fc65h02 antibody, wu:fe06e07 antibody, wu:fz79f03 antibody, zgc:136397 antibody, zgc:158541 antibody, fbx5 antibody, Fbxo5 antibody, ACYPI007854 antibody, fbxo5 antibody, F-box protein 5 antibody, F-box only protein 5 antibody, F-box protein 5 L homeolog antibody, F-box protein 5 S homeolog antibody, FBXO5 antibody, Fbxo5 antibody, fbxo5 antibody, fbxo5.L antibody, fbxo5.S antibody
    背景
    Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
    途径
    Mitotic G1-G1/S Phases
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