KRT8 抗体 (AA 343-357)
Quick Overview for KRT8 抗体 (AA 343-357) (ABIN3024645)
抗原
See all KRT8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 343-357
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无交叉反应
- 大鼠
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纯化方法
- Protein G affinity chromatography
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免疫原
- A keratin preparation from a human carcinoma was used as the immunogen for this Cytokeratin 8 antibody. The epitope of this Ab is located between amino acids 343-357.
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亚型
- IgG1 kappa
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anti-Keratin 8 (KRT8) antibody
KRT8 适用: 人, 小鼠 WB, IHC, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal M20 unconjugated
anti-Keratin 8 (KRT8) (AA 92-393) antibodyKRT8 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) (Ser726), (Ser731) antibodyKRT8 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) antibodyKRT8 适用: 人, 大鼠, 斑马鱼 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal H1 unconjugated
anti-Keratin 8 (KRT8) (pSer432) antibodyKRT8 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) (AA 91-381) antibodyKRT8 适用: 人, 大鼠, 猴, 犬 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B12 unconjugated
anti-Keratin 8 (KRT8) (AA 1-483) antibodyKRT8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) antibodyKRT8 适用: 人, Cow, 兔, Pig, 绵羊 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal B22-1 unconjugated
anti-Keratin 8 (KRT8) (AA 391-483) antibodyKRT8 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8A5D12 unconjugated
anti-Keratin 8 (KRT8) (Middle Region) antibodyKRT8 适用: 人, 大鼠, 小鼠, Cow, 兔, Pig, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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The optimal dilution of the Cytokeratin 8 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Cytokeratin 8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- KRT8 (Keratin 8 (KRT8))
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别名
- Cytokeratin 8
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背景
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.
抗原
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