Chromogranin A 抗体
Quick Overview for Chromogranin A 抗体 (ABIN3024525)
抗原
See all Chromogranin A (CHGA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human protein was used as the immunogen for the Chromogranin A antibody cocktail.
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亚型
- IgG
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anti-Chromogranin A (CHGA) (AA 15-148) antibody
CHGA 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) (AA 24-154) antibodyCHGA 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CGA-413 unconjugated
anti-Chromogranin A (CHGA) (AA 278-457) antibodyCHGA 适用: 人 IHC, FACS, WB, ELISA 宿主: 小鼠 Monoclonal 5H10B12 unconjugated
anti-Chromogranin A (CHGA) (AA 87-252) antibodyCHGA 适用: 人 FACS, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1A6B6 unconjugated
anti-Chromogranin A (CHGA) (AA 17-446) antibodyCHGA 适用: Pig IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) (AA 278-457) antibodyCHGA 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 1B7A4 unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人, 小鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人, 大鼠, 小鼠, 猴, Pig IHC, StM 宿主: 小鼠 Monoclonal LK2H10 unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人 IHC, StM 宿主: 兔 Monoclonal CHGA-1731R unconjugated Recombinant Antibody
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应用备注
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Optimal dilution of the Chromogranin A antibody cocktail should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Chromogranin A antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Chromogranin A (CHGA)
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别名
- Chromogranin A
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背景
- Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
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途径
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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