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CD45RB 抗体

This anti-CD45RB antibody is a 小鼠 单克隆 antibody detecting CD45RB in FACS, IF, WB 和 IHC (p). Suitable for 人.
产品编号 ABIN3024427
发货至: 中国

Quick Overview for CD45RB 抗体 (ABIN3024427)

抗原

See all CD45RB 抗体
CD45RB

适用

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宿主

  • 61
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  • 18
小鼠

克隆类型

  • 101
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  • 1
单克隆

标记

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This CD45RB antibody is un-conjugated

应用范围

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  • 45
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Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

PD7-26
  • 纯化方法

    Protein G purified

    免疫原

    Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western Blot: 0.5-1 μg/mL, FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the CD45RB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    CD45RB

    别名

    CD45RB

    背景

    CD45RB is one of the four major isoforms of CD45.  The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved.  Clone PD7/26 antibody is specific to CD45RB, and stains most leukemias and lymphomas. This CD45RB antibody is often used in a cocktail with clone 2B11, which will bind to all CD45 isoforms (< a href=../cd45-antibody-cocktail-2b11-pd726-v2243>Cat No V2243).

    基因ID

    5788
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