CD45RB 抗体
Quick Overview for CD45RB 抗体 (ABIN3024394)
抗原
See all CD45RB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Extract from the hepatoma cell line L428 was used as immunogen for this CD45RB antibody.
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亚型
- IgG3 kappa
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anti-CD45RB (CD45RB) antibody
CD45RB 适用: 人, Non-Human Primate FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-55 unconjugated
anti-CD45RB (CD45RB) antibody (FITC)CD45RB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-55 FITC
anti-CD45RB (CD45RB) antibody (APC)CD45RB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-55 APC
anti-CD45RB (CD45RB) antibody (PE)CD45RB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-55 PE
anti-CD45RB (CD45RB) antibody (PE)CD45RB 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal C363-16A PE
anti-CD45RB (CD45RB) antibodyCD45RB 适用: 人, 猴, 猕猴, 兔 FACS, IHC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM55 unconjugated
anti-CD45RB (CD45RB) antibodyCD45RB 适用: 人, 猴, 猕猴, 兔 FACS, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-55 unconjugated
anti-CD45RB (CD45RB) antibody (APC)CD45RB 适用: 人, 猴, 猕猴 FACS, IHC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-55 APC
anti-CD45RB (CD45RB) antibodyCD45RB 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal C363-16A unconjugated
anti-CD45RB (CD45RB) antibody (Biotin)CD45RB 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal C363-16A Biotin
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应用备注
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Due to variation in protocol and secondary antibody used, the CD45RB antibody may need to be titered for optimal performance.
1. FFPE staining requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells,Immunofluorescence: 0.5-1 μg/mL,Immunohistology (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- The CD45RB antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- CD45RB
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别名
- CD45RB
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背景
- CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin. This antigen is also expressed on the surface of cells of myeloid lineage.
抗原
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