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Calponin 抗体

The 小鼠 单克隆 anti-Calponin antibody is suitable to detect Calponin in samples from 人 和 大鼠. It has been validated for IHC (p) 和 IF.
产品编号 ABIN3024264
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Calponin 抗体 (ABIN3024264)

抗原

Calponin

适用

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人, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This Calponin antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

克隆位点

CLPN5-1
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human protein was used as the immunogen for the Calponin antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Optimal dilution of the Calponin antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    Calponin

    背景

    Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
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