Melanoma 抗体
This anti-Melanoma antibody is a 小鼠 单克隆 antibody detecting Melanoma in IHC (p), IF 和 FACS. Suitable for 人 和 马.
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Quick Overview for Melanoma 抗体 (ABIN3024241)
抗原
Melanoma
适用
人, 马
宿主
小鼠
克隆类型
单克隆
标记
This Melanoma antibody is un-conjugated
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
克隆位点
NKI-beteb
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无交叉反应
- 大鼠
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纯化方法
- Protein G affinity chromatography
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免疫原
- Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
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亚型
- IgG2b kappa
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anti-Melanoma antibody
适用: 人, 犬, 小鼠 IHC (p), IF 宿主: 小鼠 Monoclonal PNL2 unconjugated
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应用备注
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Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Melanoma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Melanoma
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背景
- By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
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基因ID
- 6490
抗原
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