Liver Arginase 抗体 (C-Term)
Quick Overview for Liver Arginase 抗体 (C-Term) (ABIN3024173)
抗原
See all Liver Arginase (ARG1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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纯化方法
- Protein G affinity chromatography
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免疫原
- A C-terminal recombinant protein fragment from ARG1 was used as the immunogen for the Arginase 1 antibody.
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亚型
- IgG3 kappa
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应用备注
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Optimal dilution of the Arginase 1 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Arginase 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Liver Arginase (ARG1) (Arginase, Liver (ARG1))
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别名
- Arginase 1
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背景
- Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [RefSeq]
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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