ZAP70 抗体
Quick Overview for ZAP70 抗体 (ABIN3024076)
抗原
See all ZAP70 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant full-length human protein was used as the immunogen for the ZAP70 antibody cocktail.
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亚型
- IgG
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anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody
ZAP70 适用: 人 WB, ICC, ICFC 宿主: 小鼠 Monoclonal ZAP-03 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibodyZAP70 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibodyZAP70 适用: 人 IHC, FACS, ELISA, IF, StM, Coat 宿主: 小鼠 Monoclonal ZAP70-2035 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibodyZAP70 适用: 人 WB, FACS, ELISA 宿主: 小鼠 Monoclonal 1F3G9 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 337-600) antibodyZAP70 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 1-254) antibodyZAP70 适用: 人 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal 2F3-2 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibodyZAP70 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibodyZAP70 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6D9C2 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 1-300) antibodyZAP70 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibodyZAP70 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the ZAP70 antibody cocktail should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the ZAP70 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
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别名
- ZAP70
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背景
- ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
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途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
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