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CD56 抗体 (Extracellular, Transmembrane Domain)

This 小鼠 单克隆 antibody specifically detects CD56 in FACS 和 IHC (p). It exhibits reactivity toward 人 和 大鼠.
产品编号 ABIN3023927
发货至: 中国
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Room 801-803
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Quick Overview for CD56 抗体 (Extracellular, Transmembrane Domain) (ABIN3023927)

抗原

See all CD56 (NCAM1) 抗体
CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

适用

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人, 大鼠

宿主

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  • 1
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小鼠

克隆类型

  • 308
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单克隆

标记

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This CD56 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

质量等级

Carrier-free

克隆位点

SPM128
  • 抗原表位

    • 24
    • 16
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    Extracellular, Transmembrane Domain

    原理

    CD56 Antibody (azide and preservative free)

    纯化方法

    Protein G affinity chromatography

    免疫原

    A membrane preparation of small cell lung carcinoma was used as the immunogen for this CD56 antibody. This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM.

    亚型

    IgG1, kappa
  • 应用备注

    The optimal dilution of the CD56 antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the CD56 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

    别名

    CD56

    背景

    Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135 kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

    UniProt

    P13591
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