S100A9 抗体
Quick Overview for S100A9 抗体 (ABIN3023909)
抗原
See all S100A9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer's cells of the liver and Langerhan's cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Human peripheral blood monocyte components were used as the immunogen for the S100A9 antibody.
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亚型
- IgG1 kappa
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anti-S100 Calcium Binding Protein A9 (S100A9) (Internal Region) antibody
Verified S100A9 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) antibodyS100A9 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) antibodyS100A9 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) antibodyS100A9 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 51-114) antibodyS100A9 适用: 人 ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) antibodyS100A9 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1C22 unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) antibodyS100A9 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C10 unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) antibodyS100A9 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal S100A9-1075 unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 2-113) antibodyS100A9 适用: 大鼠, 小鼠 WB, IHC, ELISA, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A9 (S100A9) (AA 2-114) antibodyS100A9 适用: 人 WB, IHC, ELISA, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the S100A9 antibody should be determined by the researcher.
1. For staining of formalin-fixed, paraffin-embedded tissues, digest with Trypsin at 1 mg/mL PBS, 15 min at RT\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the S100A9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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别名
- S100A9
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背景
- This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer's cells of the liver and Langerhan's cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.
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途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
抗原
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