Macrophages 抗体
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北京 101111
Quick Overview for Macrophages 抗体 (ABIN3023906)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer s cells of the liver and Langerhan s cells of the skin. Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and pro-monocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). LN-5 selectively stains human sebaceous glands in formalin-fixed, paraffin-embedded skin samples. Undifferentiated sebocyte progenitors are negative, and only sebocytes from the onset of their differentiation reveal positive cytoplasmic staining. Since there are very few selective and easy-to-use markers of sebaceous glands, LN-5 antibody can offer a simple and relatively specific way to detect human sebocytes from the onset of their.
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纯化方法
- PEG precipitation
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免疫原
- Human peripheral blood mononuclear cells were used as the immunogen for the Macrophage antibody.
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亚型
- IgM
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anti-Macrophages (pan) antibody
适用: 大鼠 IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal Ki-M2R unconjugated
anti-Macrophages antibody适用: Pig FACS, IHC (fro), WB, IP 宿主: 小鼠 Monoclonal BA4D5 unconjugated
anti-Macrophages antibody适用: 人, 大鼠, Pig, Cat, Cow, 犬, Baboon, 山羊, 豚鼠, 马, 兔, Cynomolgus, 猕猴 FACS, IHC (fro), IHC (p), IHC 宿主: 小鼠 Monoclonal MAC387 unconjugated
anti-Macrophages (pan) antibody适用: 小鼠 FACS, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal BM8 unconjugated
anti-Macrophages (pan) antibody适用: 小鼠 IHC (fro) 宿主: 大鼠 Monoclonal MOMA1 unconjugated
anti-Macrophages antibody (FITC)适用: 人, 大鼠, Pig, Cat, Cow, 犬, Baboon, 山羊, 豚鼠, 马, 兔, Cynomolgus, 猕猴 FACS 宿主: 小鼠 Monoclonal MAC387 FITC
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应用备注
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Optimal dilution of the Macrophage antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Formalin-fixed): 1-2 μg/mL for 30 minutes at RT (1),Not suitable for frozen tissues
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Macrophage antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Macrophages
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别名
- Macrophage
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背景
- This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer s cells of the liver and Langerhan s cells of the skin. Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and pro-monocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). LN-5 selectively stains human sebaceous glands in formalin-fixed, paraffin-embedded skin samples. Undifferentiated sebocyte progenitors are negative, and only sebocytes from the onset of their differentiation reveal positive cytoplasmic staining. Since there are very few selective and easy-to-use markers of sebaceous glands, LN-5 antibody can offer a simple and relatively specific way to detect human sebocytes from the onset of their.
抗原
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