LAT 抗体 (Cytoplasmic Domain)
(2 references)Quick Overview for LAT 抗体 (Cytoplasmic Domain) (ABIN302069)
抗原
See all LAT 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Cytoplasmic Domain
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原理
- Anti-LAT Purified
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特异性
- The antibody LAT-01 reacts with an intracellular epitope of LAT, a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
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无交叉反应
- 小鼠
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交叉反应 （详细）
- Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT.
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亚型
- IgG1
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anti-Linker For Activation of T Cells (LAT) antibody
LAT 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells (LAT) (pTyr191) antibodyLAT 适用: 人 WB, ELISA, IHC, IP, IF 宿主: 兔 Monoclonal 2E4 unconjugated Recombinant Antibody
anti-Linker For Activation of T Cells (LAT) (AA 1-233) antibodyLAT 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3B11-1D5 unconjugated
anti-Linker For Activation of T Cells (LAT) (AA 30-237) antibodyLAT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells (LAT) (pTyr161) antibodyLAT 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells (LAT) (Cytoplasmic Domain) antibodyLAT 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal LAT1111 unconjugated
anti-Linker For Activation of T Cells (LAT) (AA 25-221) antibodyLAT 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells (LAT) (AA 33-227) antibodyLAT 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells (LAT) (Tyr255) antibodyLAT 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells (LAT) (AA 1-233) antibodyLAT 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized.
Flow cytometry: Recommended dilution: 1-4 μg/mL, intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.
Western blotting: Recommended dilution: 1-2 μg/mL. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).
: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).
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: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).
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- LAT (Linker For Activation of T Cells (LAT))
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别名
- LAT
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背景
- Linker for activation of T cells,LAT (linker for activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.,IMD52
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基因ID
- 27040
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UniProt
- O43561
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway
抗原
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