TUBG1 抗体 (AA 434-449)
(11 references)Quick Overview for TUBG1 抗体 (AA 434-449) (ABIN302067)
抗原
See all TUBG1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 434-449
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原理
- Anti-gamma-Tubulin Purified
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特异性
- The antibody TU-32 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
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交叉反应 （详细）
- Animals, Protozoa, Plants
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449
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亚型
- IgG1
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anti-Tubulin, gamma 1 (TUBG1) (C-Term) antibody
TUBG1 适用: 人, 大鼠, 小鼠, Cow, 小鸡, 植物, Protozoa, Pig WB, ICC, ICFC 宿主: 小鼠 Monoclonal TU-30 unconjugated
anti-Tubulin, gamma 1 (TUBG1) antibodyTUBG1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Tubulin, gamma 1 (TUBG1) (AA 29-184) antibodyTUBG1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, gamma 1 (TUBG1) antibodyTUBG1 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 3E4 unconjugated
anti-Tubulin, gamma 1 (TUBG1) (AA 1-451) antibodyTUBG1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, gamma 1 (TUBG1) (N-Term) antibodyTUBG1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, gamma 1 (TUBG1) (AA 434-449), (C-Term) antibodyTUBG1 适用: 人, 大鼠, 小鼠, 小鸡, 植物, Protozoa, Pig WB, IF 宿主: 小鼠 Monoclonal TU-30 unconjugated
anti-Tubulin, gamma 1 (TUBG1) (C-Term) antibodyTUBG1 适用: 人, 非洲爪蟾, Rodents WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, gamma 1 (TUBG1) antibodyTUBG1 适用: 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, gamma 1 (TUBG1) (AA 152-451) antibodyTUBG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.
Immunocytochemistry: Methanol/acetone fixation required. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).
: "Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines." in: Journal of neuropathology and experimental neurology, Vol. 65, Issue 5, pp. 465-77, (2006) (PubMed).
: "Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 12, pp. 7243-53, (2006) (PubMed).
: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, (2004) (PubMed).
: "Gamma-tubulin in chicken erythrocytes: changes in localization during cell differentiation and characterization of cytoplasmic complexes." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 223, Issue 2, pp. 229-40, (2002) (PubMed).
: "Association of brain gamma-tubulins with alpha beta-tubulin dimers." in: The Biochemical journal, Vol. 365, Issue Pt 3, pp. 889-95, (2002) (PubMed).
: "Monoclonal antibody KN-01 against the heavy chain of kinesin." in: Folia biologica, Vol. 48, Issue 2, pp. 77-9, (2002) (PubMed).
: "Localization of gamma-tubulin in interphase and mitotic cells of a unicellular eukaryote, Giardia intestinalis." in: European journal of cell biology, Vol. 79, Issue 6, pp. 438-45, (2000) (PubMed).
: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: The Plant cell, Vol. 12, Issue 3, pp. 433-42, (2000) (PubMed).
: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, (1999) (PubMed).
: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).
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: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).
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- TUBG1 (Tubulin, gamma 1 (TUBG1))
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别名
- gamma-Tubulin
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背景
- Tubulin gamma 1,The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation.,TUBG
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基因ID
- 7283
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UniProt
- P23258
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途径
- Microtubule Dynamics, M Phase
抗原
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