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DYKDDDDK Tag 抗体

This 小鼠单克隆 antibody specifically detects DYKDDDDK Tag in WB, IP 和 IF. It has been mentioned in 11+ publications

Western blot analysis of extracts of normal 293T cells, 293T transfected with CD2 Protein and 293T transfected with CD95 Protein, using Mouse anti DDDDK-Tag mAb antibody (ABIN3020558, ABIN3020559, ABIN3020560 and ABIN1512923) at 1:10000 dilution. (DYKDDDDK Tag 抗体)

Immunofluorescence analysis of 293T-BC-Flag-GFP-C cells using Mouse anti DDDDK-Tag mAb (ABIN3020558, ABIN3020559, ABIN3020560 and ABIN1512923) at dilution of 1:100 (40x lens). (DYKDDDDK Tag 抗体)

Immunoprecipitation analysis of 200 μg extracts of 293Ttransfected with GSK3B Protein cells using 3 μg Mouse anti DDDDK-Tag mAb antibody (ABIN3020558, ABIN3020559, ABIN3020560 and ABIN1512923). (DYKDDDDK Tag 抗体)

Immunofluorescence analysis of 293T-BC-Flag-GFP-N cells using Mouse anti DDDDK-Tag mAb (ABIN3020558, ABIN3020559, ABIN3020560 and ABIN1512923) at dilution of 1:100 (40x lens). (DYKDDDDK Tag 抗体)

Immunofluorescence analysis of 293T cells using Mouse anti DDDDK-Tag mAb (ABIN3020558, ABIN3020559, ABIN3020560 and ABIN1512923) at dilution of 1:100 (40x lens). (DYKDDDDK Tag 抗体)

Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with IRF1 Protein, GSK3B Protein,and SERPINB1 Protein ,293F transfected with COPB2 Protein using Mouse anti DDDDK-Tag mAb antibody (ABIN3020558, ABIN3020559, ABIN3020560 and ABIN1512923) at 1:1000 dilution. (DYKDDDDK Tag 抗体)

6 images

(11 references)

产品编号 ABIN3020559

发货至: 中国

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Quick Overview for DYKDDDDK Tag 抗体 (ABIN3020559)

抗原

DYKDDDDK Tag

适用

请咨询

宿主

小鼠

克隆类型

单克隆

标记

This DYKDDDDK Tag antibody is un-conjugated

应用范围

Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

序列

DYKDDDDK

纯化方法

Affinity purification

免疫原

Fusion protein of human DYKDDDDK-Tag

亚型

IgG
anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated

anti-DYKDDDDK Tag antibody
适用: 标记 WB, ELISA, IP, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated

anti-DYKDDDDK Tag antibody
适用: 人 WB 宿主: 兔 Polyclonal RB0493 unconjugated

anti-DYKDDDDK Tag antibody (HRP)
WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A8E5 HRP

anti-DYKDDDDK Tag antibody
WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody (HRP)
WB, ELISA, IP 宿主: 兔 Polyclonal HRP

应用备注

WB: 1:5000, IF: 1:2000, IP: 1:100

限制

仅限研究用
缓冲液

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze / thaw cycles

储存条件

-20 °C / -80 °C
Liang, Li, Jin, Xu, Pi, Liu, Zhu, Zhang, Luan, Gong, Zhang: "Pharmacological effect of human melanocortin-2 receptor accessory protein 2 variants on hypothalamic melanocortin receptors." in: Endocrine, Vol. 61, Issue 1, pp. 94-104, (2019) (PubMed).

Liu, Xu, Zhu, Zhang, Chen, Zhou, Chen, Lin: "The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice." in: Journal of integrative plant biology, Vol. 60, Issue 2, pp. 173-188, (2018) (PubMed).

Wang, Yao, Wang, Ma, Tong, Wang, Bao, Jiang, Yang, Zhang, Xu, Wang, Zhang, Wang: "Overexpression of VpEIFP1, a novel F-box/Kelch-repeat protein from wild Chinese Vitis pseudoreticulata, confers higher tolerance to powdery mildew by inducing thioredoxin z proteolysis." in: Plant science : an international journal of experimental plant biology, Vol. 263, pp. 142-155, (2018) (PubMed).

He, Wang, Higgins, Yu, Zong, Lu, Zhang, Liang: "MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice." in: The Plant cell, Vol. 28, Issue 8, pp. 1879-93, (2017) (PubMed).

Jing, Zhou, Fang, Ding, Wang, Ke, Chen, Xiao: "DExD/H-Box Helicase 36 Signaling via Myeloid Differentiation Primary Response Gene 88 Contributes to NF-κB Activation to Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection." in: Frontiers in immunology, Vol. 8, pp. 1365, (2017) (PubMed).

Liu, Xin, Wang, Wang, Wang, Zhang, Yang, Zhang, Zhang, Wang, Xu, Duan, Xia: "Estrogen receptors in granulosa cells govern meiotic resumption of pre-ovulatory oocytes in mammals." in: Cell death & disease, Vol. 8, Issue 3, pp. e2662, (2017) (PubMed).

Ye, Chen, Li, Zhao, He, Zohaib, Song, Deng, Zhang, Chen, Cao: "Japanese Encephalitis Virus NS5 Inhibits Type I Interferon (IFN) Production by Blocking the Nuclear Translocation of IFN Regulatory Factor 3 and NF-κB." in: Journal of virology, Vol. 91, Issue 8, (2017) (PubMed).

Jing, Fang, Ding, Wang, Hao, Gao, Ke, Chen, Xiao: "Porcine Reproductive and Respiratory Syndrome Virus nsp1α Inhibits NF-κB Activation by Targeting the Linear Ubiquitin Chain Assembly Complex." in: Journal of virology, Vol. 91, Issue 3, (2017) (PubMed).

Chen, Ye, Ashraf, Li, Wei, Wan, Zohaib, Song, Chen, Cao: "MicroRNA-33a-5p Modulates Japanese Encephalitis Virus Replication by Targeting Eukaryotic Translation Elongation Factor 1A1." in: Journal of virology, Vol. 90, Issue 7, pp. 3722-34, (2016) (PubMed).

Fan, Zhang, Qian, Qian, Wu, Chen, Li: "Swine TRIM21 restricts FMDV infection via an intracellular neutralization mechanism." in: Antiviral research, Vol. 127, pp. 32-40, (2016) (PubMed).

Li, Liu, Yang, Fu, Zhao, Shen, Miao, Rayner, Chavanas, Zhu, Britt, Tang, McVoy, Luo: "Human Cytomegalovirus Infection Dysregulates the Localization and Stability of NICD1 and Jag1 in Neural Progenitor Cells." in: Journal of virology, Vol. 89, Issue 13, pp. 6792-804, (2015) (PubMed).
抗原

DYKDDDDK Tag

别名

FLAG tag

物质类

Tag

背景

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(DYKDDDDK) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DYKDDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DYKDDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DYKDDDDK peptide is more likely to be accessible to antibodies. A DYKDDDDK-Tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against3xDYKDDDDK DYKDDDDK sequence and recognizes the (3x)DYKDDDDK peptide and detects DYKDDDDK-Tagged proteins.