电话:: +86 (0512) 65829739
传真:: +86 (010) 6788 5057
电子邮件:: orders@antibodies-online.cn

我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

HLA-DR1 抗体 (PE)

This anti-HLA-DR1 antibody is a 小鼠单克隆 antibody detecting HLA-DR1 in FACS. Suitable for 人.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR1-empty (MEM-267) PE antibody (concentration in sample 9 μg/mL). (HLA-DR1 抗体 (PE))

Western blotting analysis of HLA-DR1 in Raji (A) and Jurkat (B) cell lines using MEM-267 antibody. (HLA-DR1 抗体 (PE))

Flow cytometry multicolor surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR1-empty (MEM-267) PE antibody (concentration in sample 9 μg/mL) and anti-human HLA-DR (L243) APC antibody (10 μL reagent / 100 μL of peripheral whole blood). (HLA-DR1 抗体 (PE))

Separation of human HLA-DR1 (empty) positive HLA-DR positive cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR1-empty (MEM-267) PE antibody (concentration in sample 9 μg/mL). (HLA-DR1 抗体 (PE))

4 images

产品编号 ABIN302033

发货至: 中国

Quick Overview for HLA-DR1 抗体 (PE) (ABIN302033)

抗原

HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This HLA-DR1 antibody is conjugated to PE

应用范围

Flow Cytometry (FACS)

克隆位点

MEM-267

原理

Anti-HLA-DR1 (empty) PE

特异性

The antibody MEM-267 specifically binds extracellularly to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.

亚型

IgG2b
anti-MHC Class II HLA-DR1 (HLA-DR1) antibody
HLA-DR1 适用: 人 FACS, ELISA, WB 宿主: 小鼠 Monoclonal MEM-267 unconjugated

anti-MHC Class II HLA-DR1 (HLA-DR1) antibody (PE)
HLA-DR1 适用: 人 FACS, ELISA, WB 宿主: 小鼠 Monoclonal MEM-267 PE

应用备注

Flow cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms. Recommended dilution: 1-5 μg/mL.

限制

仅限研究用
浓度

0.1 mg/mL

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
抗原

HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))

别名

HLA-DR1

背景

HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.

You are here: