HLA-DR1 抗体 (PE)
This anti-HLA-DR1 antibody (ABIN302033) is a Mouse Monoclonal antibody detecting HLA-DR1 in FACS. Suitable for Human.
Quick Overview for HLA-DR1 抗体 (PE) (ABIN302033)
抗原
HLA-DR1
(MHC Class II HLA-DR1 (HLA-DR1))
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This HLA-DR1 antibody is conjugated to PE
应用范围
Flow Cytometry (FACS)
克隆位点
MEM-267
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原理
- Anti-HLA-DR1 (empty) PE
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特异性
- The antibody MEM-267 specifically binds extracellularly to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).
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交叉反应 （详细）
- Human
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纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.
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亚型
- IgG2b
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anti-MHC Class II HLA-DR1 (HLA-DR1) antibody
HLA-DR1 适用: 人 FACS, ELISA, WB 宿主: 小鼠 Monoclonal MEM-267 unconjugated
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应用备注
- Flow cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms. Recommended dilution: 1-5 μg/mL.
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Do not freeze.
Avoid prolonged exposure to light. -
储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))
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别名
- HLA-DR1
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背景
- HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
抗原
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