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CPE 抗体 (AA 42-453)

CPE 适用: 人 WB, ELISA, IP, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN299519
发货至: 中国
  • 抗原 See all CPE 抗体
    CPE (Carboxypeptidase E (CPE))
    抗原表位
    • 15
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 42-453
    适用
    • 53
    • 34
    • 32
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 52
    • 1
    山羊
    克隆类型
    • 52
    • 1
    多克隆
    标记
    • 23
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CPE antibody is un-conjugated
    应用范围
    • 38
    • 19
    • 13
    • 13
    • 11
    • 8
    • 6
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)
    特异性
    Recognizes human Carboxypeptidase E. Cross-reactivity: Shows less than 1 % cross-reactivity with rhCPA1, rmCPA4 and rhCPB1 in ELISA and Western Blot.
    纯化方法
    Affinity purified
    免疫原
    NS0-derived recombinant human Carboxypeptidase E (aa42-453).
    亚型
    IgG
    Top Product
    Discover our top product CPE Primary Antibody
  • 应用备注
    Approved: ELISA (0.1 - 0.2 μg/mL), Flo, IP, WB (0.1 - 0.2 μg/mL)

    Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. ELISA: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~1 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~5 ng/lane under reducing and non-reducing conditions. Flow Cytometry: (A172 cells): For intracellular staining cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μLto 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents. The detection limit is ~1 ng/well. Immunoprecipitation: Used to immunoprecipitate rhCPE from conditioned media of transfected NSO cells.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile PBS
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS, 5 % trehalose.
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    -20 °C
    储存方法
    Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
  • 抗原
    CPE (Carboxypeptidase E (CPE))
    别名
    Carboxypeptidase e / CPE (CPE 产品)
    背景
    Name/Gene ID: CPE
    Subfamily: Metallopeptidase M14
    Family: Exopeptidase

    Synonyms: CPE, Carboxypeptidase E, CPH, Carboxypeptidase H, Enkephalin convertase
    基因ID
    1363
    UniProt
    P16870
    途径
    Peptide Hormone Metabolism, Synaptic Membrane
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