CPE 抗体 (AA 42-453)
Quick Overview for CPE 抗体 (AA 42-453) (ABIN299519)
抗原
See all CPE 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 42-453
-
特异性
- Recognizes human Carboxypeptidase E. Cross-reactivity: Shows less than 1 % cross-reactivity with rhCPA1, rmCPA4 and rhCPB1 in ELISA and Western Blot.
-
纯化方法
- Affinity purified
-
免疫原
- NS0-derived recombinant human Carboxypeptidase E (aa42-453).
-
亚型
- IgG
-
-
anti-Carboxypeptidase E (CPE) (AA 318-476) antibody
CPE 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (Internal Region) antibodyCPE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) antibodyCPE 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (AA 36-85) antibodyCPE 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 兔, 斑马鱼, Bat, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (C-Term) antibodyCPE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (AA 201-300) antibodyCPE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (C-Term) antibodyCPE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (AA 252-363) antibodyCPE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (AA 271-320) antibodyCPE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (AA 257-476) antibodyCPE 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Approved: ELISA (0.1 - 0.2 μg/mL), Flo, IP, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. ELISA: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~1 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~5 ng/lane under reducing and non-reducing conditions. Flow Cytometry: (A172 cells): For intracellular staining cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μLto 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents. The detection limit is ~1 ng/well. Immunoprecipitation: Used to immunoprecipitate rhCPE from conditioned media of transfected NSO cells. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Sterile PBS
-
浓度
- Lot specific
-
缓冲液
- Lyophilized from PBS, 5 % trehalose.
-
注意事项
- Avoid freeze/thaw cycles.
-
储存条件
- -20 °C
-
储存方法
- Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
-
-
- CPE (Carboxypeptidase E (CPE))
-
别名
- Carboxypeptidase e / CPE
-
背景
-
Name/Gene ID: CPE
Subfamily: Metallopeptidase M14
Family: Exopeptidase
Synonyms: CPE, Carboxypeptidase E, CPH, Carboxypeptidase H, Enkephalin convertase -
基因ID
- 1363
-
UniProt
- P16870
-
途径
- Peptide Hormone Metabolism, Synaptic Membrane
抗原
-
