MBP Tag 抗体
Our Local Distributor
北京 101111
Quick Overview for MBP Tag 抗体 (ABIN299389)
抗原
宿主
克隆类型
标记
应用范围
-
-
特异性
- MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag. To date this antibody has reacted with all MBP tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins
-
纯化方法
- Purified IgG
-
免疫原
-
The MBP epitope tag recombinant protein.
Type of Immunogen: Recombinant protein
-
-
anti-MBP Tag antibody
WB 宿主: 兔 Polyclonal RB55937 unconjugated
anti-MBP Tag antibody适用: 标记 WB, ELISA, IP, ICC, IHC, FACS 宿主: 小鼠 Monoclonal 3G1A3 unconjugated
anti-MBP Tag antibodyWB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-MBP Tag antibodyWB 宿主: 小鼠 Monoclonal 8K2 unconjugated
anti-MBP Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 5B10B11 unconjugated
-
-
应用备注
-
Approved: ELISA, WB
Usage: Anti-MBP is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~42 kD representing MBP. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
溶解方式
- deionized water
-
浓度
- Lot specific
-
缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- MBP Tag
-
物质类
- Tag
抗原
-
