CDHR5 抗体 (AA 29-479)
Quick Overview for CDHR5 抗体 (AA 29-479) (ABIN298718)
抗原
See all CDHR5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 29-479
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特异性
- Recognizes mouse MUCDHL. In direct ELISAs and Western Blots, this antibody shows approximately 5 % cross-reactivity with rhMUCDHL.
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纯化方法
- Immunoaffinity purified
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免疫原
- Purified, NS0-derived rmMUCDHL (rmMUCDHL, aa 29 - 479, Accession # NP_082345).
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亚型
- IgG
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应用备注
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Approved: ELISA, Flo, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Western Blot: Can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for r mMUCDHL is approximately 1 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Tested in flow cytometry using primary kidney cells. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Direct ELISA: Can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for r mMUCDHL is approximately 0.5 ng/well. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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