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Tumor Protein p73 抗体 (N-Term)

This anti-Tumor Protein p73 antibody is a 小鼠 单克隆 antibody detecting Tumor Protein p73 in WB, IHC, IHC (p), IP 和 ICC. Suitable for 人 和 小鼠.
产品编号 ABIN298091
发货至: 中国

Quick Overview for Tumor Protein p73 抗体 (N-Term) (ABIN298091)

抗原

See all Tumor Protein p73 (TP73) 抗体
Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

适用

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人, 小鼠

宿主

  • 125
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小鼠

克隆类型

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单克隆

标记

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This Tumor Protein p73 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

克隆位点

5B429
  • 抗原表位

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    N-Term

    特异性

    Recognizes all of the TAp73 isoforms i. e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kD and 45 kD, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.

    纯化方法

    Protein G purified

    免疫原

    Full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.

    亚型

    IgG
  • 应用备注

    Approved: ICC, IHC, IHC-P (5 μg/mL), IP, WB (2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS containing 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

    别名

    TP73 / p73

    背景

    Name/Gene ID: TP73

    Synonyms: TP73, p53-like transcription factor, p73, p53-related protein, Tumor protein p73

    基因ID

    7161

    UniProt

    O15350

    途径

    Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus
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