Tumor Protein p73 抗体 (N-Term)
Quick Overview for Tumor Protein p73 抗体 (N-Term) (ABIN298091)
抗原
See all Tumor Protein p73 (TP73) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- Recognizes all of the TAp73 isoforms i. e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kD and 45 kD, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.
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纯化方法
- Protein G purified
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免疫原
- Full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
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亚型
- IgG
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应用备注
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Approved: ICC, IHC, IHC-P (5 μg/mL), IP, WB (2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS containing 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))
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别名
- TP73 / p73
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背景
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Name/Gene ID: TP73
Synonyms: TP73, p53-like transcription factor, p73, p53-related protein, Tumor protein p73 -
基因ID
- 7161
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UniProt
- O15350
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途径
- Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus
抗原
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