Neural Wiskott-Aldrich syndrome protein (WASL) (AA 21-270) 抗体
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- 抗原 See all Neural Wiskott-Aldrich syndrome protein (WASL) 抗体
- Neural Wiskott-Aldrich syndrome protein (WASL)
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抗原表位
- AA 21-270
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- 特异性
- Selected for its ability to recognize human N-WASP in the applications listed.
- 纯化方法
- Affinity purified
- 免疫原
- E. coli-derived rhN-WASP (aa21-270).
- 亚型
- IgG
- Top Product
- Discover our top product WASL Primary Antibody
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- 应用备注
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 1.0 μg/mL with the appropriate secondary reagents to detect N-WASP. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect human N-WASP on A431 human epidermoid carcinoma cells stimulated with EGF. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human N-WASP. The detection limit for rhN-WASP is approximately 1.0 ng/well. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- deionized water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized.
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