Neural Wiskott-Aldrich syndrome protein (WASL) (AA 21-270) 抗体
Quick Overview for Neural Wiskott-Aldrich syndrome protein (WASL) (AA 21-270) 抗体 (ABIN297874)
抗原
See all Neural Wiskott-Aldrich syndrome protein (WASL) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 21-270
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特异性
- Selected for its ability to recognize human N-WASP in the applications listed.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived rhN-WASP (aa21-270).
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亚型
- IgG
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应用备注
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 1.0 μg/mL with the appropriate secondary reagents to detect N-WASP. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect human N-WASP on A431 human epidermoid carcinoma cells stimulated with EGF. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human N-WASP. The detection limit for rhN-WASP is approximately 1.0 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- Neural Wiskott-Aldrich syndrome protein (WASL)
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别名
- WASL / N-WASP
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背景
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Name/Gene ID: WASL
Synonyms: WASL, N-WASP, NWASP, Wiskott-Aldrich syndrome-like -
基因ID
- 8976
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UniProt
- O00401
抗原
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