SOX15 抗体
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- 抗原 See all SOX15 抗体
- SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))
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适用
- 人
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This SOX15 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- 特异性
- Selected for its ability to recognize human SOX15 in the applications listed.
- 纯化方法
- Affinity purified
- 免疫原
- E. coli-derived rhSOX15.
- 亚型
- IgG
- Top Product
- Discover our top product SOX15 Primary Antibody
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anti-SRY (Sex Determining Region Y)-Box 15 (SOX15) (AA 1-233) antibody
SOX15 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2C1 unconjugated
anti-SRY (Sex Determining Region Y)-Box 15 (SOX15) (AA 142-191) antibodySOX15 适用: 人, 豚鼠, 大鼠, Cow, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 15 (SOX15) (AA 96-125) antibodySOX15 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB20745 unconjugated
anti-SRY (Sex Determining Region Y)-Box 15 (SOX15) (AA 152-165), (C-Term) antibodySOX15 适用: 人 WB 宿主: 兔 Polyclonal RB51530 unconjugated
anti-SRY (Sex Determining Region Y)-Box 15 (SOX15) (AA 1-233) antibodySOX15 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 15 (SOX15) (N-Term) antibodySOX15 适用: 人, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 15 (SOX15) (Internal Region) antibodyVerified SOX15 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 15 (SOX15) (Middle Region) antibodySOX15 适用: 人, 小鼠, 豚鼠, 大鼠, Cow, 马, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 20 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 1 % cross-reactivity with rhSOX1, -2, -5, -6, -7, -10, -12, -14, -15, -17, and rhSOX21. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect SOX15 in NTera2 teratocarcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 0.3 ng/well. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- deionized water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized.
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