Lipin 1 抗体 (AA 448-574)
Quick Overview for Lipin 1 抗体 (AA 448-574) (ABIN297253)
抗原
See all Lipin 1 (LPIN1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 448-574
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特异性
- Selected for its ability to recognize human Lipin 1 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 2 % cross-reactivity with rhLipin2 and rhLipin3.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived rhLipin 1 (aa448-574).
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亚型
- IgG
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anti-Lipin 1 (LPIN1) (AA 729-924) antibody
LPIN1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lipin 1 (LPIN1) (AA 201-300) antibodyLPIN1 适用: 大鼠, 小鼠 IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lipin 1 (LPIN1) (AA 741-890) antibodyLPIN1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lipin 1 (LPIN1) (AA 218-267) antibodyLPIN1 适用: 人, 大鼠, 小鼠, 犬, 豚鼠, 马, 兔, Bat, Hamster, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lipin 1 (LPIN1) (AA 792-890) antibodyLPIN1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D9 unconjugated
anti-Lipin 1 (LPIN1) (AA 501-600) antibodyLPIN1 适用: 大鼠 IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lipin 1 (LPIN1) (AA 241-340) antibodyLPIN1 适用: 大鼠, 小鼠 IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lipin 1 (LPIN1) (AA 741-890) antibodyLPIN1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Lipin 1 (LPIN1) (N-Term) antibodyLPIN1 适用: 人, 大鼠, 小鼠, 犬, 豚鼠, 马, 兔, Cow, Pig, Saccharomyces cerevisiae, 绵羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lipin 1 (LPIN1) (AA 300-400), (Internal Region) antibodyLPIN1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, ICC, WB
Usage: Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect human Lipin 1 on human MSC-derived adipocytes. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Lipin 1. The detection limit for rhLipin 1 is approximately 2 ng/lane under non-reducing and reducing conditions. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Lipin 1. The detection limit for rhLipin 1 is approximately 0.1 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- Lipin 1 (LPIN1)
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别名
- LPIN1 / Lipin 1
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背景
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Name/Gene ID: LPIN1
Synonyms: LPIN1, Lipin-1, Lipin 1, PAP1, KIAA0188 -
基因ID
- 23175
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UniProt
- Q14693
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途径
- Monocarboxylic Acid Catabolic Process
抗原
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