HAPLN4 抗体
Quick Overview for HAPLN4 抗体 (ABIN297170)
抗原
See all HAPLN4 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Selected for its ability to recognize human HAPLN4 in the applications listed. In Western Blots, this antibody shows less than 1 % cross-reactivity with rhHAPLN1.
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纯化方法
- Affinity purified
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免疫原
- NS0-derived rhHAPLN4.
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亚型
- IgG
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anti-Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4) (AA 30-134) antibody
HAPLN4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G5 unconjugated
anti-Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4) (AA 30-134) antibodyHAPLN4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3H1 unconjugated
anti-Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4) (AA 30-134) antibodyHAPLN4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4) (AA 251-300) antibodyHAPLN4 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4) (C-Term) antibodyHAPLN4 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ICC, IP, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human HAPLN4. The detection limit for rhHAPLN4 is approximately 10 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhHAPLN4 from conditioned media of transfected NS0 cells. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect HAPLN4 on SHSY5Y human neuroblastoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4))
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别名
- HAPLN4
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背景
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Name/Gene ID: HAPLN4
Synonyms: HAPLN4, BRAL2, KIAA1926, Brain link protein 2 -
基因ID
- 404037
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UniProt
- Q86UW8
抗原
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